Journal article

Resistance toBacillus thuringiensisCry1Ac toxin requires mutations in twoPlutella xylostellaATP-binding cassette transporter paralogs

Zhaoxia Liu, Shu Fu, Xiaoli Ma, Simon W Baxter, Liette Vasseur, Lei Xiong, Yuping Huang, Guang Yang, Shijun You, Minsheng You

PLoS Pathogens | Public Library of Science (PLoS) | Published : 2020

Abstract

The diamondback moth, Plutella xylostella, is a cosmopolitan pest and the first species to develop field resistance to toxins from the gram-positive bacterium Bacillus thuringiensis (Bt). Although previous work has suggested that mutations of ATP-binding cassette transporter subfamily C2 (ABCC2) or C3 (ABCC3) genes can confer Cry1Ac resistance, here we reveal that P. xylostella requires combined mutations in both PxABCC2 and PxABCC3 to achieve high-level Cry1Ac resistance, rather than simply a mutation of either gene. We identified natural mutations of PxABCC2 and PxABCC3 that concurrently occurred in a Cry1Ac-resistant strain (Cry1S1000) of P. xylostella, with a mutation (RA2) causing the m..

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University of Melbourne Researchers

Grants

Awarded by National Natural Science Foundation of China


Awarded by State Key Laboratory of Ecological Pest Control for Fujian-Taiwan Crops


Awarded by Joint International Research Laboratory of Ecological Pest Control (Educational Ministry of China)


Awarded by "111" Program in China


Awarded by Science and Technology Major Project of the Fujian Province


Funding Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31972271), the operational funds of the State Key Laboratory of Ecological Pest Control for Fujian-Taiwan Crops (KEA17002A), the Joint International Research Laboratory of Ecological Pest Control (Educational Ministry of China) (KJG18018A), the "111" Program in China (KRA16001A), and Science and Technology Major Project of the Fujian Province (2018NZ0002-1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.