Journal article
MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth
Z Guo, S Kang, D Chen, Q Wu, S Wang, W Xie, X Zhu, SW Baxter, X Zhou, JL Jurat-Fuentes, Y Zhang
Plos Genetics | Published : 2015
Open access
Abstract
Insecticidal crystal toxins derived from the soil bacterium Bacillus thuringiensis (Bt) are widely used as biopesticide sprays or expressed in transgenic crops to control insect pests. However, large-scale use of Bt has led to field-evolved resistance in several lepidopteran pests. Resistance to Bt Cry1Ac toxin in the diamondback moth, Plutella xylostella (L.), was previously mapped to a multigenic resistance locus (BtR-1). Here, we assembled the 3.15 Mb BtR-1 locus and found high-level resistance to Cry1Ac and Bt biopesticide in four independent P. xylostella strains were all associated with differential expression of a midgut membrane-bound alkaline phosphatase (ALP) outside this locus and..
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Funding Acknowledgements
This work was supported by the National Natural Science Foundation of China (30871659; 30471159; 31201532), the 863 Program (2012AA101502), the Agricultural Science and Technology Innovation Program, the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables, and the Biotechnology Risk Assessment Grant Program competitive grants Nos. 2010-33522-21700 and 2014-33522-22215 from the USDA National Institute of Food and Agriculture. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.