Genomic islands of divergence in hybridizing Heliconius butterflies identified by large-scale targeted sequencing
Nicola J Nadeau, Annabel Whibley, Robert T Jones, John W Davey, Kanchon K Dasmahapatra, Simon W Baxter, Michael A Quail, Mathieu Joron, Richard H Ffrench-Constant, Mark L Blaxter, James Mallet, Chris D Jiggins
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES | ROYAL SOC | Published : 2012
Heliconius butterflies represent a recent radiation of species, in which wing pattern divergence has been implicated in speciation. Several loci that control wing pattern phenotypes have been mapped and two were identified through sequencing. These same gene regions play a role in adaptation across the whole Heliconius radiation. Previous studies of population genetic patterns at these regions have sequenced small amplicons. Here, we use targeted next-generation sequence capture to survey patterns of divergence across these entire regions in divergent geographical races and species of Heliconius. This technique was successful both within and between species for obtaining high coverage of alm..View full abstract
Awarded by Biotechnology and Biological Sciences Research Council
Awarded by Medical Research Council
Awarded by Natural Environment Research Council
This work was funded primarily by a Leverhulme Trust award to C.D.J. and a BBSRC grant to J.M., C.D.J. and M. B. Funding for A. W. was by the European Research Council starting grant 'MimEvol' to M.J. and R.T.J. was funded by a Leverhulme grant to R.Hf.-C. The GenePool is funded by awards from the MRC, NERC, the Scottish Universities' Life Sciences Alliance and the Darwin Trust of Edinburgh. We thank the Direccion General Forestal y de Fauna Silvestre (Ministerio de Agricultura) in Peru and ANAM in Panama for collecting permits and the staff at the Smithsonian Tropical Research Institute insectaries in Gamboa Panama. We also thank Alexi Balmuth for performing the SureSelect target enrichment and the sequencing teams at the GenePool, Edinburgh (SureSelect sequencing) and the Wellcome Trust Sanger Institute (preparing and sequencing fosmids). Thanks are also due to Paul Wilkinson for help with repeat masking, Jamie Walters for annotating additional BACs and Vincent Plagnol for helpful discussions and informatics support.