Journal article

Characterization and expression of the cytochrome P450 gene family in diamondback moth, Plutella xylostella (L.)

Liying Yu, Weiqi Tang, Weiyi He, Xiaoli Ma, Liette Vasseur, Simon W Baxter, Guang Yang, Shiguo Huang, Fengqin Song, Minsheng You

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2015

Abstract

Cytochrome P450 monooxygenases are present in almost all organisms and can play vital roles in hormone regulation, metabolism of xenobiotics and in biosynthesis or inactivation of endogenous compounds. In the present study, a genome-wide approach was used to identify and analyze the P450 gene family of diamondback moth, Plutella xylostella, a destructive worldwide pest of cruciferous crops. We identified 85 putative cytochrome P450 genes from the P. xylostella genome, including 84 functional genes and 1 pseudogene. These genes were classified into 26 families and 52 subfamilies. A phylogenetic tree constructed with three additional insect species shows extensive gene expansions of P. xyloste..

View full abstract

University of Melbourne Researchers

Grants

Awarded by National Natural Science Foundation of China


Awarded by National Key Project of Fundamental Scientific Research ("973" Programs) in China


Funding Acknowledgements

We are grateful to Dr. David R. Nelson (the International Cytochrome Nomenclature Committee), for nomenclature of the DBM P450 genes, and to Dr. Xianchun Li for his advice on sequence analysis. We thank Yanchun You for her advice on primer design and the RT-PCR experiment, and Jianyu Li and Lishuang Zheng for DBM rearing and sampling. We appreciate Dr. Mark Goettel for his comments and suggestions on the manuscript revision. The work is supported by National Natural Science Foundation of China (No. 31320103922 and No. 31230061), and National Key Project of Fundamental Scientific Research ("973" Programs, No. 2011CB100404) in China. L. V. is supported by the Minjiang Scholar Fellowship, and S. B. is supported by the Jinshan Scholar Fellowship and the Australian Research Council.