Construction and characterisation of near-isogenic Plutella xylostella (Lepidoptera: Plutellidae) strains resistant to Cry1Ac toxin
Xun Zhu, Yanyuan Lei, Yanjv Yang, Simon W Baxter, Jianhong Li, Qingjun Wu, Shaoli Wang, Wen Xie, Zhaojiang Guo, Wei Fu, Youjun Zhang
PEST MANAGEMENT SCIENCE | JOHN WILEY & SONS LTD | Published : 2015
BACKGROUND: Resistance to insecticidal Bacillus thuringiensis (Bt) toxins has arisen in multiple populations of the worldwide Brassica pest Plutella xylostella (L.). To help elucidate the mechanism of resistance to Bt Cry1Ac toxin in a population from Florida, two pairs of near-isogenic lines (NILs) were developed. RESULTS: NILs were generated using either backcross or recombinant inbred line methodologies and evaluated for near-isogenicity with inter-simple-sequence-repeat (ISSR) markers. Backcross line BC6F4 maintained a similar level of Cry1Ac resistance to parental strain DBM1Ac-R (>5000-fold) yet showed 98.24% genetic similarity to the susceptible parental strain DBM1Ac-S. Single-pair b..View full abstract
Awarded by National Natural Science Foundation of China
Awarded by 863 Programme
Awarded by Special Fund for Agroscientific Research in the Public Interest
This research was supported by the Agricultural Science and Technology Innovation Programme (ASTIP), the National Natural Science Foundation of China (30871659), the 863 Programme (2012AA101502), the Special Fund for Agroscientific Research in the Public Interest (201203038), and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables. Special thanks go to Prof. Juan Luis Jurat-Fuentes (Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN) for comments on an earlier draft. XZ, YL, SWB and YZ designed the study, XZ, YL, YY and ZG performed the experiments, XZ, YL and YY analysed the data, XZ wrote the manuscript, SWB, JL, QW, SW, WX and WF revised the manuscript and all authors read and approved the final version of the manuscript. The authors have declared that no conflicts of interest exist.