Journal article

Linkage Mapping and Comparative Genomics Using Next-Generation RAD Sequencing of a Non-Model Organism

Simon W Baxter, John W Davey, J Spencer Johnston, Anthony M Shelton, David G Heckel, Chris D Jiggins, Mark L Blaxter

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2011

Abstract

Restriction-site associated DNA (RAD) sequencing is a powerful new method for targeted sequencing across the genomes of many individuals. This approach has broad potential for genetic analysis of non-model organisms including genotype-phenotype association mapping, phylogeography, population genetics and scaffolding genome assemblies through linkage mapping. We constructed a RAD library using genomic DNA from a Plutella xylostella (diamondback moth) backcross that segregated for resistance to the insecticide spinosad. Sequencing of 24 individuals was performed on a single Illumina GAIIx lane (51 base paired-end reads). Taking advantage of the lack of crossing over in homologous chromosomes i..

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University of Melbourne Researchers

Grants

Awarded by Biotechnology and Biological Sciences Research Council (BBSRC)


Awarded by Medical Research Council (MRC)


Awarded by Natural Environment Research Council (NERC)


Awarded by Biotechnology and Biological Sciences Research Council


Awarded by Medical Research Council


Awarded by Natural Environment Research Council


Funding Acknowledgements

This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) [grant numbers G00661X, F021135 to JWD and MLB and RG47616 to SWB and CDJ]; the Medical Research Council (MRC) [grant number G0900740 to MLB and the GenePool], the Max-Planck-Gesellschaft [DGH]; and Natural Environment Research Council (NERC) [grant number R8/H10/56 to MLB and the GenePool]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.