Journal article

Lessons from Drosophila: Engineering Genetic Sexing Strains with Temperature-Sensitive Lethality for Sterile Insect Technique Applications

Thu NM Nguyen, Amanda Choo, Simon W Baxter

INSECTS | MDPI | Published : 2021


A major obstacle of sterile insect technique (SIT) programs is the availability of robust sex-separation systems for conditional removal of females. Sterilized male-only releases improve SIT efficiency and cost-effectiveness for agricultural pests, whereas it is critical to remove female disease-vector pests prior to release as they maintain the capacity to transmit disease. Some of the most successful Genetic Sexing Strains (GSS) reared and released for SIT control were developed for Mediterranean fruit fly (Medfly), Ceratitis capitata, and carry a temperature sensitive lethal (tsl) mutation that eliminates female but not male embryos when heat treated. The Medfly tsl mutation was generated..

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University of Melbourne Researchers


Awarded by Hermon Slade Foundation

Awarded by Horticulture Innovation Australia

Funding Acknowledgements

This research was funded by the Hermon Slade Foundation grant number HSF 18-6. A.C. was funded by Horticulture Innovation Australia grant numbers MT13059 and FF18002, with research and development levy funds from the vegetable, apple and pear, citrus, strawberry, table grape, cherry and summerfruit industries, with co-investment from South Australian Research and Development Institute (SARDI) and Primary Industries and Regions South Australia (PIRSA).