Journal article
Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial–mesenchymal transition
A Shafiq, W Suwakulsiri, A Rai, M Chen, DW Greening, HJ Zhu, R Xu, RJ Simpson
Proteomics | Published : 2021
Abstract
Epithelial–mesenchymal transition (EMT) describes an evolutionary conserved morphogenic process defined by loss of epithelial characteristics and acquisition of mesenchymal phenotype, and altered patterns of intercellular communication, leading to functional changes in cell migration and invasion. In this regard, we have previously reported that oncogenic H-Ras induced EMT in Madin-Darby Canine Kidney (MDCK) cells (21D1 cells) trigger changes in the protein distribution pattern in cells, exosomes, and soluble protein factors (secretome) which modulate the tumor microenvironment. Here, we report that shed microvesicles (also termed microparticles/ectosomes) secreted from MDCK cells following ..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
A.S., R.X., W.S, M.C., D.W.G, A.R. and R.J.S acknowledge funding support from La Trobe University. A.S. and W.S. are supported by La Trobe University Postgraduate Scholarships. This work was supported, in part, by the National Health & Medical Research Council (NHMRC) of Australia for grants #280913 (H-J.Z.) and #1057741 and #1139489 (D.W.G.). We acknowledge La Trobe University Comprehensive Proteomics Platform and Bioimaging Platforms infrastructure support.