A bacterial stimulation assay for bronchoalveolar lavage immune cells from young children with cystic fibrosis
Haipei Liu, Sohinee Sarkar, Kristy Azzopardi, Sophie Day, Serene Yeow, Sarath Ranganathan, Philip Sutton
Scandinavian Journal of Immunology | WILEY | Published : 2021
Cystic Fibrosis (CF) is primarily a progressive lung disease, characterized by chronic pulmonary infections with opportunistic pathogens. Such infections typically commence early in life, producing an inflammatory response marked by IL-8 chemokine production and neutrophilic infiltration, major contributory factors in CF progression. Studying this inflammation, especially early in life, is critical for developing new strategies for preventing or slowing disruption to the structural integrity of the CF airways. However, evaluating the immune responses of bronchoalveolar lavage (BAL) cells from children with CF faces technical challenges, including contamination carried from the lung due to pr..View full abstract
This work was supported by grants from 65km for Cystic Fibrosis and the Victorian Government's Operational Infrastructure Support Program. HL was supported by the Shanghai High--level Local University Innovation Team Support Program.