Journal article

Distribution of microRNA profiles in pre-clinical and clinical forms of murine and human prion disease

Lesley Cheng, Camelia Quek, Xia Li, Shayne A Bellingham, Laura J Ellett, Mitch Shambrook, Saima Zafar, Inga Zerr, Victoria A Lawson, Andrew F Hill

COMMUNICATIONS BIOLOGY | NATURE RESEARCH | Published : 2021

Abstract

Prion diseases are distinguished by long pre-clinical incubation periods during which prions actively propagate in the brain and cause neurodegeneration. In the pre-clinical stage, we hypothesize that upon prion infection, transcriptional changes occur that can lead to early neurodegeneration. A longitudinal analysis of miRNAs in pre-clinical and clinical forms of murine prion disease demonstrated dynamic expression changes during disease progression in the affected thalamus region and serum. Serum samples at each timepoint were collected whereby extracellular vesicles (EVs) were isolated and used to identify blood-based biomarkers reflective of pathology in the brain. Differentially express..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by Robert Koch Institute through Federal Ministry of Health


Funding Acknowledgements

This study was funded by the National Health and Medical Research Council of Australia (to A.F.H GNT1002349; GNT1041413; GNT1132604) and the Australian CJD Support Group Network Memorial Award in memory of Lorraine Seabrook. L.C. was awarded the 2019 Australian CJD Support Group Network Memorial Award in memory of Adrian Chesterton, Norma Crawley and Danilo Banzon. I.Z. received funds by the Robert Koch Institute through funds from the Federal Ministry of Health (Grant no. 1369-341). We wish to thank the families for providing serum samples for this study. We thank Viviane Grewe for providing technical assistance in organising the serum samples for this international collaboration, and the La Trobe University Statistics Consultancy Platform for statistical advice and analysis.