Renal Denervation Promotes Atherosclerosis in Hypertensive Apolipoprotein E-Deficient Mice Infused with Angiotensin II
Yutang Wang, Tam N Dinh, Alexander Nield, Smriti M Krishna, Kate Denton, Jonathan Golledge
FRONTIERS IN PHYSIOLOGY | FRONTIERS MEDIA SA | Published : 2017
Objective: To determine the effect of renal denervation (RDN) on the severity of atherosclerosis and aortic aneurysm in hypertensive mice. Methods: Hypertension, atherosclerosis and aortic aneurysm were induced by subcutaneous infusion of angiotensin II (1 μg/kg/min) for 28 days in apolipoprotein E-deficient mice. RDN was conducted using combined surgical and local chemical denervation. The norepinephrine concentration in the kidney was measured by high-performance liquid chromatography. Blood pressure was measured by the tail-cuff method. Atherosclerosis was assessed by Sudan IV staining of the aortic arch. The aortic diameter was measured by the morphometric method. The mRNA expression of ..View full abstract
Awarded by National Health and Medical Research Council
This work was funded by grants from the National Health and Medical Research Council (1062671, 1079369,1079193, 1063476, 1021416, and 1000967) and Queensland Government. JG is supported by a Practitioner Fellowship from the National Health and Medical Research Council (1117061).