A Peptide Antagonist of Thrombospondin-1 Promotes Abdominal Aortic Aneurysm Progression in the Angiotensin II-Infused Apolipoprotein-E-Deficient Mouse
Smriti M Krishna, Sai Wang Seto, Roby J Jose, Erik Biros, Corey S Moran, Yutang Wang, Paula Clancy, Jonathan Golledge
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | LIPPINCOTT WILLIAMS & WILKINS | Published : 2015
OBJECTIVE: Interaction of the activating sequence in thrombospondin-1 (TSP-1) with the conserved sequence (leucine-serine-lysine-leucine [LSKL]) in the latency-associated peptide region of latent transforming growth factor (TGF)-β complex is important in regulating TGF-β1 activity. We aimed to assess the effect of blocking peptide LSKL on the progression of pre-established abdominal aortic aneurysm in angiotensin II-infused apolipoprotein E-deficient (ApoE(-/-)) mice. APPROACH AND RESULTS: Abdominal aortic aneurysm was established in 3-month-old male ApoE(-/-) mice with subcutaneous infusion of angiotensin II for 28 days. After this, mice received LSKL peptide or control SLLK (serine-leucine..View full abstract
Awarded by National Health and Medical Research Council
Awarded by National Health and Medical Research Council (Centre of Research Excellence)
Awarded by National Heart Foundation, Australia
This work was supported by project grants from the National Health and Medical Research Council (IDs 1079193, 1079369, 1021416, 1003707). J. Golledge is supported by grants from the National Health and Medical Research Council (project grants 1079193, 1079369, 1063476, 1022752, 1021416, 1020955, 1003707; Centre of Research Excellence 1000967; fellowship 1019921), and The Office of Health and Medical Research, Queensland Government. Dr S.W. Seto is a recipient of a fellowship from the National Heart Foundation, Australia (PF12B6825). The funding bodies played no role in generation of the data presented in this publication.