Impaired Acetylcholine-Induced Endothelium-Dependent Aortic Relaxation by Caveolin-1 in Angiotensin II-Infused Apolipoprotein-E (ApoE(-/-)) Knockout Mice
Sai Wang Seto, Smriti M Krishna, Hongyou Yu, David Liu, Surabhi Khosla, Jonathan Golledge
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2013
OBJECTIVE: The angiotensin II (AngII)-infused apolipoprotein E-deficient (ApoE(-/-)) mouse model is widely used to study atherosclerosis and abdominal aortic aneurysm. An increase in blood pressure has been reported in this model however the underlying mechanism has not been fully explored. In this study, we investigated whether vasomotor dysfunction develops in AngII-infused ApoE(-/-) mice and the underlying mechanism involved. METHODS: ApoE(-/-) mice were infused with vehicle (distilled water) or AngII subcutaneously for 14 days. Blood pressure and heart rate were measured using the non-invasive tail cuff method. Aortic vascular reactivity and expression of key proteins (endothelial nitric..View full abstract
This work was partially supported by the James Cook University Research Infrastructure Block Grant (RIBG) and School Administered Grant (SAG) Scheme (2011), by a grant from the British United Provident Association (BUPA) Foundation and by an Australia Government National Health and Medical Research Council (NHMRC) project grant. SW Seto is a recipient of a training fellowship from Australia Government National Health and Medical Research Council and S Khosla was supported by the summer scholarship from the National Heart Foundation, Australia. J Golledge is supported by fellowships from the Queensland Government and National Health and Medical Research Council, Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.