Proteomic and genomic analyses suggest the association of apolipoprotein C1 with abdominal aortic aneurysm
Joseph V Moxon, Dawei Liu, Corey S Moran, David J Crossman, Smriti M Krishna, Ponlapat Yonglitthipagon, Theophilus I Emeto, Dylan R Morris, Matthew P Padula, Jason P Mulvenna, Catherine M Rush, Jonathan Golledge
PROTEOMICS CLINICAL APPLICATIONS | WILEY-V C H VERLAG GMBH | Published : 2014
PURPOSE: Abdominal aortic aneurysm (AAA) is an important cause of mortality in the elderly. Mouse models are widely used to investigate AAA pathogenesis but their suitability for biomarker discovery is unexplored. EXPERIMENTAL DESIGN: We conducted a three-phase study. Phase 1: Aortas from angiotensin-II-infused apolipoprotein E deficient (ApoE(-/-) ) mice with and without AAA were assessed via iTRAQ and analyzed in silico to identify potential circulating markers. Microarray data from ApoE(-/-) mice and human patients were analyzed in parallel. Phase 2: Putative markers were compared between datasets to shortlist common candidates. Phase 3: The relationship of two shortlisted markers and AAA..View full abstract
JVM thanks Dr. Yutang Wang for useful discussions. We thank the Queensland Tropical Health Alliance for research infrastructure. This work is supported by grants from James Cook University (JVM), the Australian National Health and Medical Research Council (NHMRC), The Townsville Hospital Private Practice Trust Fund, and the Queensland Government (JG, Principal Investigator). JG holds a Practitioner Fellowship from the NHMRC, Australia, and a Senior Clinical Research Fellowship from the Queensland Government.