Journal article

Development of a two-stage limb ischemia model to better simulate human peripheral artery disease

Smriti M Krishna, Safraz Mohamed Omer, Jiaze Li, Susan K Morton, Roby J Jose, Jonathan Golledge



Peripheral arterial disease (PAD) develops due to the narrowing or blockage of arteries supplying blood to the lower limbs. Surgical and endovascular interventions are the main treatments for advanced PAD but alternative and adjunctive medical therapies are needed. Currently the main preclinical experimental model employed in PAD research is based on induction of acute hind limb ischemia (HLI) by a 1-stage procedure. Since there are concerns regarding the ability to translate findings from this animal model to patients, we aimed to develop a novel clinically relevant animal model of PAD. HLI was induced in male Apolipoprotein E (ApoE-/-) deficient mice by a 2-stage procedure of initial gradu..

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Awarded by National Health and Medical Research Council, Australia

Funding Acknowledgements

This research was funded by a Faculty Administered Grant and a Research Infrastructure Block Grant from James Cook University and funding from the Queensland Government. JG holds a Practitioner Fellowship from the National Health and Medical Research Council, Australia (1117061) and a Senior Clinical Research Fellowship from the Queensland Government. SMO was supported by funding from the Graduate Research School and College of Medicine, James Cook University. We would like to acknowledge with thanks the help of Prof. Zoltan Sarnyai (James Cook University) who provided access to Open Field Test assessment facility, Dr. Joseph Moxon (James Cook University) who assisted with the LME used to analyse data from the exercise study and Dr. Pacific Huynh (James Cook University) who provided the mouse images in Figs. 1A and 5A.