Journal article

Increased Pathogenicity and Virulence of Middle East Respiratory Syndrome Coronavirus Clade B In Vitro and In Vivo

Yanqun Wang, Jing Sun, Xiaobo Li, Airu Zhu, Wenda Guan, De-Qiang Sun, Mian Gan, Xuefeng Niu, Jun Dai, Lu Zhang, Zhaoyong Zhang, Yongxia Shi, Shuxiang Huang, Chris Ka Pun Mok, Zifeng Yang, Zhongfang Wang, Wenjie Tan, Yimin Li, Ling Chen, Rongchang Chen Show all

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2020

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe acute respiratory disease in humans. MERS-CoV strains from early epidemic clade A and contemporary epidemic clade B have not been phenotypically characterized to compare their abilities to infect cells and mice. We isolated the clade B MERS-CoV ChinaGD01 strain from a patient infected during the South Korean MERS outbreak in 2015 and compared the phylogenetics and pathogenicity of MERS-CoV EMC/2012 (clade A) and ChinaGD01 (clade B) in vitro and in vivo Genome alignment analysis showed that most clade-specific mutations occurred in the orf1ab gene, including mutations that were predicted to be potential glycosylation sites...

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University of Melbourne Researchers

Grants

Awarded by Guangdong Provincial Department of Science and Technology


Awarded by National Key Research and Development Program of China


Awarded by National Science and Technology Major Project


Awarded by National Natural Science Foundation of China


Awarded by State Key Laboratory of Respiratory Disease


Awarded by Guangzhou Medical University High-level University Innovation Team Training Program (Guangzhou Medical University release [2017])


Awarded by National Key Technology RD Program


Awarded by Guangdong Science and Technology Foundation


Funding Acknowledgements

This research was supported in part by grants from the Guangdong Provincial Department of Science and Technology (2018B020207013), the National Key Research and Development Program of China (2018YFC1200100 and 2016YFC1202701), the National Science and Technology Major Project (2018ZX10301403-001-003 and 2018ZX10101002), the National Natural Science Foundation of China (81772191, 81702047, 91842106, and 8181101118), the Thousand Talents Plan Award of China 2015 and the State Key Laboratory of Respiratory Disease (SKLRD-QN-201715 and SKLRD-QN-201912), the Guangzhou Medical University High-level University Innovation Team Training Program (Guangzhou Medical University release [2017] no. 159), the National Key Technology R&D Program (2018YFC1311900), and the Guangdong Science and Technology Foundation (2019B030316028).