A novel role for milk fat globule-EGF factor 8 protein (MFGE8) in the mediation of mouse sperm-extracellular vesicle interactions.
Natalie A Trigg, Simone J Stanger, Wei Zhou, David A Skerrett-Byrne, Petra Sipilä, Matthew D Dun, Andrew L Eamens, Geoffry N De Iuliis, Elizabeth G Bromfield, Shaun D Roman, Brett Nixon
Proteomics | Wiley | Published : 2021
Spermatozoa transition to functional maturity as they are conveyed through the epididymis, a highly specialized region of the male excurrent duct system. Owing to their transcriptionally and translationally inert state, this transformation into fertilization competent cells is driven by complex mechanisms of intercellular communication with the secretory epithelium that delineates the epididymal tubule. Chief among these mechanisms are the release of extracellular vesicles (EV), which have been implicated in the exchange of varied macromolecular cargo with spermatozoa. Here, we describe the optimization of a tractable cell culture model to study the mechanistic basis of sperm-extracellular v..View full abstract