Journal article

Nuclear localisation of West Nile virus NS5 protein modulates host gene expression

Adam J Lopez-Denman, Daniel E Tuipulotu, Jessica B Ross, Alice M Trenerry, Peter A White, Jason M Mackenzie



The involvement of the nucleus during flavivirus infection has been observed in only a small number of cases and can be limited to primarily two viral proteins; the structural protein C and the RNA polymerase NS5. Previously we observed that by blocking nuclear transport, WNV strain Kunjin (WNVKUN) replication is severely affected and through mutation of the identified NLS in WNVKUN NS5 protein. In this study, we interrogated the potential nuclear functions of WNVKUN NS5 has on the host transcriptome, by means of RNA sequencing (RNAseq). In a direct comparison between wild type and mutant NS5, it can also be determined that the nuclear translocation of NS5 results in a significant down-regul..

View full abstract

University of Melbourne Researchers


Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

This research was supported by a Project Grant (No. 1081756) to J. M.M. from the National Health and Medical Research Council of Australia. The funding body played no role in the design of the study and collection, analysis, and interpretation of data nor the writing of the manuscript.