Assessment of the microbiome during bacteriophage therapy in combination with systemic antibiotics to treat a case of staphylococcal device infection
Andre Mu, Daniel McDonald, Alan K Jarmusch, Cameron Martino, Caitriona Brennan, Mackenzie Bryant, Gregory C Humphrey, Julia Toronczak, Tara Schwartz, Dominic Nguyen, Gail Ackermann, Anthony D'Onofrio, Steffanie A Strathdee, Robert T Schooley, Pieter C Dorrestein, Rob Knight, Saima Aslam
MICROBIOME | BMC | Published : 2021
BACKGROUND: Infectious bacterial diseases exhibiting increasing resistance to antibiotics are a serious global health issue. Bacteriophage therapy is an anti-microbial alternative to treat patients with serious bacterial infections. However, the impacts to the host microbiome in response to clinical use of phage therapy are not well understood. RESULTS: Our paper demonstrates a largely unchanged microbiota profile during 4 weeks of phage therapy when added to systemic antibiotics in a single patient with Staphylococcus aureus device infection. Metabolomic analyses suggest potential indirect cascading ecological impacts to the host (skin) microbiome. We did not detect genomes of the three pha..View full abstract
Awarded by Endeavour Research Fellowship (Australia Awards)
Awarded by QIIME2
A.M. was funded by the Endeavour Research Fellowship (Australia Awards) to collaborate with the Department of Pediatrics, UCSD, CA, USA (ERF PDR 6735_2018). R.K. and D.M. was funded by QIIME2 (NSF DBI-1565057). The following authors contributed to this work through funding from the American Gut Project: G.A., C.B., M.B., G.C.H., R.K., D.M., D.N., and J.T.