Journal article

High production yield and specific productivity of succinate from cassava starch by metabolically-engineered Escherichia coli KJ122

K Khor, A Sawisit, S Chan, S Kanchanatawee, SS Jantama, K Jantama

Journal of Chemical Technology and Biotechnology | Published : 2016

Abstract

BACKGROUND: Succinate has been widely used in chemical industries and its microbial production is a desirable option. The feasibility of bio-based succinate production on an industrial scale strongly depends on the utilization of cheaper renewable resources. The use of cassava starch may make the fermentation process of succinate more economically competitive. RESULTS: Simultaneous saccharification and fermentation (SSF) of succinate production was performed in minimal salt medium containing a cheap cassava starch. The parameters affecting succinate production from cassava starch by E. coli KJ122 were optimized. A succinate concentration of 70.1 ± 0.1 g Ll−1 was produced with a yield of 1.00..

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