Journal article

Production of succinic acid from sucrose and sugarcane molasses by metabolically engineered Escherichia coli.

Sitha Chan, Sunthorn Kanchanatawee, Kaemwich Jantama

Bioresour Technol | Published : 2012

Abstract

Sucrose-utilizing genes (cscKB and cscA) from Escherichia coli KO11 were cloned and expressed in a metabolically engineered E. coli KJ122 to enhance succinate production from sucrose. KJ122 harboring a recombinant plasmid, pKJSUC, was screened for the efficient sucrose utilization by growth-based selection and adaptation. KJ122-pKJSUC-24T efficiently utilized sucrose in a low-cost medium to produce high succinate concentration with less accumulation of by-products. Succinate concentrations of 51 g/L (productivity equal to 1.05 g/L/h) were produced from sucrose in anaerobic bottles, and concentrations of 47 g/L were produced in 10L bioreactor within 48 h. Antibiotics had no effect on the succ..

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University of Melbourne Researchers