Comparison of lipid and detergent enzyme environments for identifying inhibitors of membrane-bound energy-transducing proteins.
Elyse A Dunn, Gregory M Cook, Adam Heikal
Journal of Microbiological Methods | Published : 2016
This study compared detergent-solubilised (soluble) and lipid-reconstituted (proteoliposome) protein to establish a high-throughput method for identifying membrane protein inhibitors. We identified inhibitors of the membrane-bound type II NADH dehydrogenase with lower lipophilicity and better potency, suggesting proteoliposome systems may be advantageous over detergent-solubilised systems for respiratory membrane proteins.