An Arabidopsis lipid map reveals differences between tissues and dynamic changes throughout development
Cheka Kehelpannala, Thusitha Rupasinghe, Asher Pasha, Eddi Esteban, Thomas Hennessy, David Bradley, Berit Ebert, Nicholas J Provart, Ute Roessner
PLANT JOURNAL | WILEY | Published : 2021
Mass spectrometry is the predominant analytical tool used in the field of plant lipidomics. However, there are many challenges associated with the mass spectrometric detection and identification of lipids because of the highly complex nature of plant lipids. Studies into lipid biosynthetic pathways, gene functions in lipid metabolism, lipid changes during plant growth and development, and the holistic examination of the role of plant lipids in environmental stress responses are often hindered. Here, we leveraged a robust pipeline that we previously established to extract and analyze lipid profiles of different tissues and developmental stages from the model plant Arabidopsis thaliana. We ana..View full abstract
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Awarded by Australian Research Council Future Fellowship
We are thankful to Associate Professor Joshua Heazlewood for facilitating the collaboration with Professor Nicholas Provart, University of Toronto, Canada to implement the electronic Fluorescent Pictograph (eFP) browser to display Arabidopsis lipids. The LC-MS instrument used in the study was accessed through Metabolomics Australia, which is supported by funds from the Australian Government's National Collaborative Research Infrastructure Scheme (NCRIS), administered through Bioplatforms Australia (BPA) Ltd. CK is funded through a Research Training Program Scholarship provided by the Australian Commonwealth Government and the University of Melbourne. The project was also supported through financial support from Agilent Technologies, Australia, to CK and UR. BE is supported by an Australian Research Council Future Fellowship (FT160100276) and the University of Melbourne Botany Foundation.