Cell and fluid sampling microneedle patches for monitoring skin-resident immunity
Anasuya Mandal, Archana Boopathy, Lionel KW Lam, Kelly D Moynihan, Mary E Welch, Nitasha R Bennett, Michelle E Turvey, Nikki Thai, Jenny H Van, J Christopher Love, Paula T Hammond, Darrell J Irvine
Science Translational Medicine | AMER ASSOC ADVANCEMENT SCIENCE | Published : 2018
Important cell populations reside within tissues and are not accessed by traditional blood draws used to monitor the immune system. To address this issue at an essential barrier tissue, the skin, we created a microneedle-based technology for longitudinal sampling of cells and interstitial fluid, enabling minimally invasive parallel monitoring of immune responses. Solid microneedle projections were coated by a cross-linked biocompatible polymer, which swells upon skin insertion, forming a porous matrix for local leukocyte infiltration. By embedding molecular adjuvants and specific antigens encapsulated in nanocapsules within the hydrogel coating, antigen-specific lymphocytes can be enriched i..View full abstract
Awarded by National Cancer Institute
Awarded by U.S. Army Research Office through the Institute for Soldier Nanotechnologies
This work was supported in part by the Ragon Institute of MGH, MIT and Harvard, the Koch Institute Support (core) Grant P30-CA14051 from the National Cancer Institute, and the U.S. Army Research Laboratory and the U.S. Army Research Office through the Institute for Soldier Nanotechnologies under contract number W911NF-13-D-0001. D.J.I. is an investigator of the Howard Hughes Medical Institute.