Journal article

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

Eitan Lerner, Anders Barth, Jelle Hendrix, Benjamin Ambrose, Victoria Birkedal, Scott C Blanchard, Richard Boerner, Hoi Sung Chung, Thorben Cordes, Timothy D Craggs, Ashok A Deniz, Jiajia Diao, Jingyi Fei, Ruben L Gonzalez, Irina Gopich, Taekjip Ha, Christian A Hanke, Gilad Haran, Nikos S Hatzakis, Sungchul Hohng Show all

ELIFE | eLIFE SCIENCES PUBL LTD | Published : 2021

Abstract

Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using variou..

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Awarded by National Institutes of Health


Awarded by National Science Foundation


Awarded by Human Frontier Science Program


Awarded by European Research Council


Awarded by Deutsche Forschungsgemeinschaft


Awarded by Deutsche Forschungsgemeinschaft CIBSS


Awarded by Wellcome Trust


Awarded by Biotechnology and Biological Sciences Research Council


Awarded by Royal Society


Awarded by Nationale de la Recherche


Awarded by Agence Nationale de la Recherche


Awarded by Israel Science Foundation


Awarded by National Research Foundation of Korea


Awarded by Independent Fund Denmark


Awarded by National Key Research and Development Program of China


Awarded by UHasselt BOF fund


Awarded by KU Leuven Special Research Fund


Awarded by Carlsbergfondet


Awarded by Villum Fonden


Awarded by Novo Nordisk


Funding Acknowledgements

National Institutes of Health GM130942 Eitan Lerner Shimon WeissNational Institutes of Health GM095904 Xavier MichaletNational Institutes of Health GM079238 Scott C BlanchardNational Institutes of Health GM098859 Scott C BlanchardNational Institutes of Health GM084288 Ruben L GonzalezNational Institutes of Health GM137608 Ruben L GonzalezNational Institutes of Health GM112882 Harold D KimNational Institutes of Health GM123164 Tae-Hee LeeNational Institutes of Health GM130793 Tae-Hee LeeNational Institutes of Health GM140272 Reza VafabakhshNational Institutes of Health GM130375 Ashok A DenizNational Institutes of Health 128185 Jingyi FeiNational Institute of Diabetes and Digestive and Kidney Diseases Hoi Sung Jung Irina V GopichNational Science Foundation 1818147 Shimon WeissNational Science Foundation 1842951 Shimon WeissNational Science Foundation 2004016 Ruben L GonzalezHuman Frontier Science Program RGP0061/2019 Shimon WeissEuropean Research Council 638536 Thorben CordesEuropean Research Council 860954 Thorben CordesEuropean Research Council SMPFv2.0 Edward A LemkeEuropean Research Council 671208 Claus AM SeidelEuropean Research Council 819299 Chirlmin JooDeutsche Forschungsgemeinschaft GRK2062 Thorben CordesDeutsche Forschungsgemeinschaft SFB863 Thorben CordesDeutsche Forschungsgemeinschaft PL696/4-1 Evelyn PloetzDeutsche Forschungsgemeinschaft SPP2191 402723784 Edward A LemkeDeutsche Forschungsgemeinschaft SFB 1129 240245660 Edward A LemkeDeutsche Forschungsgemeinschaft SE 1195/21-1 (SPP 2191) Claus AM SeidelDeutsche Forschungsgemeinschaft CIBSS - EXC-2189 - project ID 390939984 Thorsten HugelWellcome Trust 110164/Z/15/Z Achillefs N KapanidisSwiss National Science Foundation Benjamin SchulerBiotechnology and Biological Sciences Research Council BB/S008896/1 Achillefs N KapanidisBiotechnology and Biological Sciences Research Council BB/T008032/1 Timothy D CraggsRoyal Society RGS\R2\180405 Timothy D CraggsRoyal Society DKR00620 Nicole C RobbRoyal Society RGF\R1\180054 Nicole C Robb AgenceNationale de la Recherche ANR-17-CE09-0026-02 Emmanuel MargeatAgence Nationale de la Recherche ANR-18-CE11-0004-02 Emmanuel MargeatAgence Nationale de la Recherche ANR-19-CE44-0009-02 Emmanuel MargeatIsrael Science Foundation 1250/19 Gilad HaranIsrael Science Foundation 3565/20 Eitan LernerNational Research Foundation of Korea 2019R1A2C2090896 Nam Ki LeeNational Research Foundation of Korea NRF-2019R1A2C2005209 Sungchul HohngNational Research Foundation of Korea NRF-2019R1A2C1089808 Seok-Cheol HongIndependent Fund Denmark 6110-00623B Viktoria BirkedalNational Key Research and Development Program of China 2018YFA0507700 Chun TangUHasselt BOF fund R-10789 Jelle HendrixDeutsche Forschungsgemeinschaft SFB1035 Don C LambMilner Fund Eitan LernerKU Leuven Special Research Fund C14/16/053 Jelle HendrixCarlsbergfondet CF16-0797 Nikos S HatzakisVillum Fonden 18333 Nikos S HatzakisNovo Nordisk NNF14CC0001 Nikos S HatzakisLudwig-Maximilians-Universitat Munchen Timothy D CraggsCarolina Cancer Center of Nanotechnology Excellence Timothy D CraggsUniversity of Melbourne Hoi Sung JungSearle Scholars Program Jingyi FeiUniversity of Zurich Richard BornerEPSRC Benjamin AmbroseThe funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.