Using Bacterial Extract along with Differential Gene Expression in Acropora millepora Larvae to Decouple the Processes of Attachment and Metamorphosis
Nachshon Siboni, David Abrego, Francois Seneca, Cherie A Motti, Nikos Andreakis, Jan Tebben, Linda L Blackall, Tilmann Harder
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2012
Biofilms of the bacterium Pseudoalteromonas induce metamorphosis of acroporid coral larvae. The bacterial metabolite tetrabromopyrrole (TBP), isolated from an extract of Pseudoalteromonas sp. associated with the crustose coralline alga (CCA) Neogoniolithon fosliei, induced coral larval metamorphosis (100%) with little or no attachment (0-2%). To better understand the molecular events and mechanisms underpinning the induction of Acropora millepora larval metamorphosis, including cell proliferation, apoptosis, differentiation, migration, adhesion and biomineralisation, two novel coral gene expression assays were implemented. These involved the use of reverse-transcriptase quantitative PCR (RT-..View full abstract
Awarded by Australian Institute of Marine Science
Funding was provided by the Australian Institute of Marine Science, Futures Project, Appropriation Fund 2233. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.