Journal article
Using bacterial extract along with differential gene expression in Acropora millepora Larvae to decouple the processes of attachment and metamorphosis
N Siboni, D Abrego, F Seneca, CA Motti, N Andreakis, J Tebben, LL Blackall, T Harder
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2012
Open access
Abstract
Biofilms of the bacterium Pseudoalteromonas induce metamorphosis of acroporid coral larvae. The bacterial metabolite tetrabromopyrrole (TBP), isolated from an extract of Pseudoalteromonas sp. associated with the crustose coralline alga (CCA) Neogoniolithon fosliei, induced coral larval metamorphosis (100%) with little or no attachment (0-2%). To better understand the molecular events and mechanisms underpinning the induction of Acropora millepora larval metamorphosis, including cell proliferation, apoptosis, differentiation, migration, adhesion and biomineralisation, two novel coral gene expression assays were implemented. These involved the use of reverse-transcriptase quantitative PCR (RT-..
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Awarded by Australian Institute of Marine Science
Funding Acknowledgements
Funding was provided by the Australian Institute of Marine Science, Futures Project, Appropriation Fund 2233. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.