Journal article

Microscale structure and function of anaerobic-aerobic granules containing glycogen accumulating organisms

RL Meyer, AM Saunders, RJX Zeng, J Keller, LL Blackall

FEMS MICROBIOLOGY ECOLOGY | OXFORD UNIV PRESS | Published : 2003

Abstract

The spatial arrangement and metabolic activity of 'Candidatus Competibacter phosphatis' was investigated in granular sludge from an anaerobic-aerobic sequencing batch reactor enriched for glycogen-accumulating organisms. In this process, the electron donor (acetate) and the electron acceptor (oxygen) were supplied sequentially in each phase. The organism, identified by fluorescence in situ hybridisation, was present throughout the granules; however, metabolic activity was limited to a 100-mum-thick layer immediately below the surface of the granules. To investigate the cause of this, oxygen microsensors and a novel microscale biosensor for volatile fatty acids were used in conjunction with c..

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University of Melbourne Researchers