Monitoring associations between clade-level variation, overall community structure and ecosystem function in enhanced biological phosphorus removal (EBPR) systems using terminal-restriction fragment length polymorphism (T-RFLP)
FR Slater, CR Johnson, LL Blackall, RG Beiko, PL Bond
WATER RESEARCH | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2010
The role of Candidatus "Accumulibacter phosphatis" (Accumulibacter) in enhanced biological phosphorus removal (EBPR) is well established but the relevance of different Accumulibacter clades to the performance of EBPR systems is unknown. We developed a terminal-restriction fragment length polymorphism (T-RFLP) technique to monitor changes in the relative abundance of key members of the bacterial community, including Accumulibacter clades, in four replicate mini-sequencing batch reactors (mSBRs) operated for EBPR over a 35-day period. The ability of the T-RFLP technique to detect trends was confirmed using fluorescence in situ hybridisation (FISH). EBPR performance varied between reactors and ..View full abstract
Awarded by ARC
This work was supported by ARC Discovery Project DP0773857 awarded to LLB, CRJ and RGB. RGB also acknowledges the support of the Canada Research Chairs programme. The authors gratefully acknowledge Mr. Jeremy Barr for his assistance with CLSM. The authors thank three anonymous reviewers for their considered criticism of the manuscript.