Journal article

Detection by polymerase chain reaction of Clostridium perfringens producing epsilon toxin in faeces and in gastrointestinal contents of goats

FA Uzal, JJ Plumb, LL Blackall, D OBoyle, WR Kelly

LETTERS IN APPLIED MICROBIOLOGY | BLACKWELL SCIENCE LTD | Published : 1996

Abstract

A polymerase chain reaction (PCR) was used to identify the gene-encoding epsilon toxin production in Clostridium perfringens types B and D in faeces and in gastrointestinal contents of goats. The samples were cultured in thioglycollate broth and centrifuged. The upper layer of the pellet was used as a template for PCR, obviating the need for DNA extraction. This technique specifically differentiated Cl. perfringens types B and D from Cl. perfringens types A and C and from Escherichia coli. When used to identify Cl. perfringens type D in samples artificially spiked with the micro-organism, the PCR detected as few as 1.4 x 10(2) cfu g-1 of sample. Gastrointestinal contents and faeces were coll..

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University of Melbourne Researchers