Microbial ecology of the equine hindgut during oligofructose-induced laminitis
Gabriel J Milinovich, Paul C Burrell, Christopher C Pollitt, Athol V Klieve, Linda L Blackall, Diane Ouwerkerk, Erika Woodland, Darren J Trott
ISME JOURNAL | NATURE PUBLISHING GROUP | Published : 2008
Alimentary carbohydrate overload is a significant cause of laminitis in horses and is correlated with drastic shifts in the composition of hindgut microbiota. Equine hindgut streptococcal species (EHSS), predominantly Streptococcus lutetiensis, have been shown to be the most common microorganisms culturable from the equine caecum prior to the onset of laminitis. However, the inherent biases of culture-based methods are estimated to preclude up to 70% of the normal caecal microbiota. The objective of this study was to evaluate bacterial population shifts occurring in the equine caecum throughout the course of oligofructose-induced laminitis using several culture-independent techniques and to ..View full abstract
This project was supported by the Rural Industries Research and Development Corporation (RIRDC) of Australia and the Animal Health Foundation (AHF), St Louis, Missouri, USA. We thank Graham Kerven of The University of Queensland's School of Land, Crop and Food Science for chromatography assistance, Lester Walters and the staff of Eagle Farm Equine Hospital for performing the surgery to place the caecal fistulas in the animals used in this study, Elena Constantinoiu of The University of Queensland's School of Veterinary Science for assistance in processing of samples, Lyle McMillen of the Department of Primary Industries and Fisheries' Animal Research Institute for technical assistance with qPCR and Christopher Owens for monitoring of animals and collection of samples.