Journal article

Investigation of candidate division TM7, a recently recognized major lineage of the domain bacteria with no known pure-culture representatives

P Hugenholtz, GW Tyson, RI Webb, AM Wagner, LL Blackall

Applied and Environmental Microbiology | AMER SOC MICROBIOLOGY | Published : 2001


A molecular approach was used to investigate a recently described candidate division of the domain Bacteria, TM7, currently known only from environmental 16S ribosomal DNA sequence data. A number of TM7-specific primers and probes were designed and evaluated. Fluorescence in situ hybridization (FISH) of a laboratory scale bioreactor using two independent TM7-specific probes revealed a conspicuous sheathed-filament morphotype, fortuitously enriched in the reactor. Morphologically, the filament matched the description of the Eikelboom morphotype 0041-0675 widely associated with bulking problems in activated-sludge wastewater treatment systems. Transmission electron microscopy of the bioreactor..

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University of Melbourne Researchers