Journal article

Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation

GR Crocetti, P Hugenholtz, PL Bond, A Schuler, J Keller, D Jenkins, LL Blackall

Applied and Environmental Microbiology | AMER SOC MICROBIOLOGY | Published : 2000


Laboratory-scale sequencing batch reactors (SBRs) as models for activated sludge processes were used to study enhanced biological phosphorus removal (EBPR) from wastewater. Enrichment for polyphosphate-accumulating organisms (PAOs) was achieved essentially by increasing the phosphorus concentration in the influent to the SBRs. Fluorescence in situ hybridization (FISH) using domain-, division-, and subdivision-level probes was used to assess the proportions of microorganisms in the sludges. The A sludge, a high-performance P-removing sludge containing 15.1% P in the biomass, was comprised of large clusters of polyphosphate-containing coccobacilli. By FISH, >80% of the A sludge bacteria were b..

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University of Melbourne Researchers