Journal article

PCR detection of Clostridium chauvoei in pure cultures and in formalin-fixed, paraffin-embedded tissues

FA Uzal, P Hugenholtz, LL Blackall, S Petray, S Moss, RA Assis, MF Miyakawa, G Carloni

VETERINARY MICROBIOLOGY | ELSEVIER | Published : 2003

Abstract

The polymerase chain reaction (PCR) was used to amplify specific segments of the 16S ribosomal RNA gene of Clostridium chauvoei, a major pathogen of ruminants. Three sets of primers were used to produce amplicons of 159, 836 and 959 base pairs (bp), respectively. The PCR was evaluated by testing clinically important strains of Clostridium, including 21 strains of C. chauvoei, five strains each of Clostridium septicum and Clostridium perfringens and two strains each of Clostridium novyi, Clostridium histolyticum and Clostridium sordellii. Both purified DNA and biomass from pure cultures of each of these microorganisms were evaluated as templates in the PCR. In addition, extracts of formalin-f..

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