Journal article

Transcriptomic Profiling of Circulating HLA-DR(-)Myeloid Cells, Compared with HLA-DR( )Myeloid Antigen-presenting Cells

Reem Saleh, Rowaida Z Taha, Varun Sasidharan Nair, Salman M Toor, Nehad M Alajez, Eyad Elkord

IMMUNOLOGICAL INVESTIGATIONS | TAYLOR & FRANCIS INC | Published : 2020

Abstract

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of cells with potent immunosuppressive functions, which can inhibit the activation of immune responses under a steady-state condition and pathological conditions. We performed transcriptomic profiling of circulating CD33+HLA-DR+ myeloid antigen-presenting cells (APCs) and CD33+HLA-DR- myeloid cells (potentially MDSCs) in healthy individuals. We sorted both subpopulations from peripheral blood mononuclear cells (PBMCs) of 10 healthy donors and performed RNA sequencing (RNA-Seq). We found that several signaling pathways associated with the positive regulation of immune responses, such as antigen presentation/processing, Fc..

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Grants

Funding Acknowledgements

We thank the staff of the Genomics Core at QBRI for performing RNA-Seq. Open Access funding provided by the Qatar National Library.