Journal article

Epigenetic regulation of immune checkpoints and T cell exhaustion markers in tumor-infiltrating T cells of colorectal cancer patients

Varun Sasidharan Nair, Reem Saleh, Salman M Toor, Rowaida Z Taha, Ayman A Ahmed, Mohamed A Kurer, Khaled Murshed, Mohamed Abu Nada, Eyad Elkord

Epigenomics | FUTURE MEDICINE LTD | Published : 2020

Abstract

Aim: To elucidate the epigenetic alterations behind the upregulation of immune checkpoints and T cell exhaustion markers in colorectal cancer (CRC) patients. Materials & methods: mRNA expressions of different immune checkpoint/exhaustion markers were analyzed by quantitative real-time reverse transcriptase PCR and epigenetic investigations were performed using bisulfite sequencing and chromatin immunoprecipitation quantitative PCR. Results: mRNA expressions of PD-1, TIM-3, CTLA-4, PD-L1 and TOX2 were significantly upregulated in CD4+ and CD8+ tumor-infiltrating lymphocytes and bulk CRC tumor tissues. Histone 3 lysine 9 trimethylation was downregulated and histone 3 lysine 4 trimethylation wa..

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Grants

Awarded by Qatar Biomedical Research Institute, Qatar Foundation


Funding Acknowledgements

This work was supported by a start-up grant (VR04) for Professor Eyad Elkord from Qatar Biomedical Research Institute, Qatar Foundation. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.