DNA methylation in the promoters of PD-L1, MMP9, ARG1, galectin-9, TIM-3, VISTA and TGF-beta genes in HLA-DR- myeloid cells, compared with HLA-DR antigen-presenting cells
Reem Saleh, Salman M Toor, Rowaida Z Taha, Dana Al-Ali, Varun Sasidharan Nair, Eyad Elkord
EPIGENETICS | TAYLOR & FRANCIS INC | Published : 2020
Myeloid cells, including antigen-presenting cells (APCs) and myeloid-derived suppressor cells (MDSCs) play opposing roles to orchestrate innate and adaptive immune responses during physiological and pathological conditions. We investigated the role of DNA methylation in regulating the transcription of inhibitory/suppressive molecules in myeloid suppressive cells (identified as CD33+HLA-DR-) in comparison to APCs. We selected a number of immune checkpoints (ICs), IC ligands, and immunosuppressive molecules that have been implicated in MDSC function, including PD-L1, TIM-3, VISTA, galectin-9, TGF-β, ARG1 and MMP9. We examined their mRNA expression levels, and investigated whether DNA methylati..View full abstract
Awarded by Qatar Biomedical Research Institute, Qatar Foundation
This work was supported by a start-up grant [VR04] for Professor Eyad Elkord from Qatar Biomedical Research Institute, Qatar Foundation.