Effective low-cost preservation of human stools in field-based studies for helminth and microbiota analysis
Katharina Stracke, Poom Adisakwattana, Suparat Phuanukoonnon, Tippayarat Yoonuan, Akkarin Poodeepiyasawat, Paron Dekumyoy, Kittipong Chaisiri, Alexandra Roth Schulze, Stephen Wilcox, Harin Karunajeewa, Rebecca J Traub, Aaron R Jex
INTERNATIONAL JOURNAL FOR PARASITOLOGY | ELSEVIER SCI LTD | Published : 2021
Molecular studies of gastrointestinal infections or microbiotas require either rapid sample processing or effective interim preservation. This is difficult in remote settings in low-income countries, where the majority of the global infectious disease burden exists. Processing or freezing of samples immediately upon collection is often not feasible and the cost of commercial preservatives is prohibitive. We compared fresh freezing (the 'gold standard' method), with low-cost chemical preservation in (i) a salt-based buffer consisting of DMSO, EDTA and NaCl (DESS) or (ii) 2.5% potassium dichromate (PD), for soil-transmitted helminth detection and microbiota characterisation in pre-school and s..View full abstract
Awarded by National Health and Medical Research Council (Australia) Career Development Fellowship
We are grateful for the ongoing work and support of the local Thai field teams, the Mae Song sub-district administration office and the Tha Song Yang district health office. We would like to thank all participants and parents without whom this study would not have been feasible. This work, including the efforts of ARJ, was funded by a National Health and Medical Research Council (Australia) Career Development Fellowship (APP1126395). KS, RJT and ARJ are supported by the Victorian State Government Operational Infrastructure Support, Australia, and Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme. HK was supported by a NHMRC Career Development Fellowship. KS is supported by a Melbourne Research Scholarship (Australian Government) and the Riady Scholarship (Victoria, Australia). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.