Journal article

A G protein-coupled receptor-like module regulates cellulose synthase secretion from the endomembrane system in Arabidopsis

Heather E McFarlane, Daniela Mutwil-Anderwald, Jana Verbancic, Kelsey L Picard, Timothy E Gookin, Anja Froehlich, David Chakravorty, Luisa M Trindade, Jose M Alonso, Sarah M Assmann, Staffan Persson

DEVELOPMENTAL CELL | CELL PRESS | Published : 2021

Abstract

Cellulose is produced at the plasma membrane of plant cells by cellulose synthase (CESA) complexes (CSCs). CSCs are assembled in the endomembrane system and then trafficked to the plasma membrane. Because CESAs are only active in the plasma membrane, control of CSC secretion regulates cellulose synthesis. We identified members of a family of seven transmembrane domain-containing proteins (7TMs) that are important for cellulose production during cell wall integrity stress. 7TMs are often associated with guanine nucleotide-binding (G) protein signaling and we found that mutants affecting the Gβγ dimer phenocopied the 7tm mutants. Unexpectedly, the 7TMs localized to the Golgi/trans-Golgi networ..

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Grants

Awarded by Australian Research Council (ARC)


Awarded by Novo Nordisk Laureate


Awarded by EMBO-LTF


Awarded by Natrual Sciences and Engineering Council (NSERC) PDF


Awarded by ARC Discovery Early Career Researcher award


Awarded by NSF


Awarded by U.S. National Science Foundation


Awarded by National Institute of GeneralMedical Sciences of the NIH


Funding Acknowledgements

Live cell imaging was conducted using instruments that are part of the Biological OpticalMicroscopyPlatform(BOMP) atUniversityofMelbourne andelectron microscopy was conducted using instruments that are part of the Melbourne Advanced Microscopy Facility. S.P. acknowledges the financial aid of an Australian Research Council (ARC) Discovery grant (DP19001941), Villum Investigator (project ID: 25915), and Novo Nordisk Laureate (NNF19OC0056076) grants. H.E.M. acknowledges an EMBO-LTF (1246-2013), Natrual Sciences and Engineering Council (NSERC) PDF (454454-2014), and an ARC Discovery Early Career Researcher award (DE170100054). J.M.A. acknowledges support from NSF grant IOS1444561, D.M.-A. acknowledges financial support fromDeutsche Forschungsgemeinschaft and T.E.G., S.M.A., and D.C. acknowledge support from the U.S. National Science Foundation (MCB-1121612, with additional support fromMCB-1715826) and fromthe National Institute of GeneralMedical Sciences of the NIH under award number R01GM126079. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.