Journal article

PRMT1-mediated H4R3me2a recruits SMARCA4 to promote colorectal cancer progression by enhancing EGFR signaling

Bing Yao, Tao Gui, Xiangwei Zeng, Yexuan Deng, Zhi Wang, Ying Wang, Dongjun Yang, Qixiang Li, Peipei Xu, Ruifeng Hu, Xinyu Li, Bing Chen, Jin Wang, Ke Zen, Haitao Li, Melissa J Davis, Marco J Herold, Hua-Feng Pan, Zhi-Wei Jiang, David CS Huang Show all

GENOME MEDICINE | BMC | Published : 2021

Abstract

BACKGROUND: Aberrant changes in epigenetic mechanisms such as histone modifications play an important role in cancer progression. PRMT1 which triggers asymmetric dimethylation of histone H4 on arginine 3 (H4R3me2a) is upregulated in human colorectal cancer (CRC) and is essential for cell proliferation. However, how this dysregulated modification might contribute to malignant transitions of CRC remains poorly understood. METHODS: In this study, we integrated biochemical assays including protein interaction studies and chromatin immunoprecipitation (ChIP), cellular analysis including cell viability, proliferation, colony formation, and migration assays, clinical sample analysis, microarray exp..

View full abstract

Grants

Awarded by National Natural Science Foundation of China NSFC


Awarded by China Postdoctoral Science Foundation


Awarded by Fundamental Research Funds for the Central Universities


Funding Acknowledgements

This work was supported by National Natural Science Foundation of China NSFC (31770809, 31970615, 81700108, and 31701191), China Postdoctoral Science Foundation (2018T110479 and 2016 M590442), and Fundamental Research Funds for the Central Universities (020814380116).