Journal article

The impact of immune checkpoint therapy on the latent reservoir in HIV-infected individuals with cancer on antiretroviral therapy.

Jillian SY Lau, James H McMahon, Celine Gubser, Ajantha Solomon, Chris YH Chiu, Ashanti Dantanarayana, Socheata Chea, Surekha Tennakoon, Jennifer M Zerbato, Jill Garlick, Vincent Morcilla, Sarah Palmer, Sharon R Lewin, Thomas A Rasmussen

AIDS | Published : 2021

Abstract

OBJECTIVE: The aim of this study was to quantify HIV-specific immunological and virological changes in people with HIV (PWH) on antiretroviral therapy (ART) with malignancy who received immune checkpoint blockade (ICB). DESIGN: An observational cohort study. METHODS: Blood samples were collected before and after four cycles of ICB in HIV-positive adults on ART. Virological assessments performed on CD4+ T cells included cell-associated unspliced HIV RNA, cell-associated HIV DNA, Tat/rev-induced limiting dilution assay (TILDA) and plasma HIV RNA using a single copy assay (SCA). Flow cytometry was used to assess the frequency of precursor exhausted T cells (Tpex) and exhausted T cells (Tex), an..

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