Journal article
CytoBas: Precision component-resolved diagnostics for allergy using flow cytometric staining of basophils with recombinant allergen tetramers
CI McKenzie, N Varese, PM Aui, BD Wines, PM Hogarth, F Thien, M Hew, JM Rolland, RE O’Hehir, MC van Zelm
Allergy European Journal of Allergy and Clinical Immunology | WILEY | Published : 2021
DOI: 10.1111/all.14832
Abstract
Background: Diagnostic tests for allergy rely on detecting allergen-specific IgE. Component-resolved diagnostics incorporate multiple defined allergen components to improve the quality of diagnosis and patient care. Objective: To develop a new approach for determining sensitization to specific allergen components that utilizes fluorescent protein tetramers for direct staining of IgE on blood basophils by flow cytometry. Methods: Recombinant forms of Lol p 1 and Lol p 5 proteins from ryegrass pollen (RGP) and Api m 1 from honeybee venom (BV) were produced, biotinylated, and tetramerized with streptavidin-fluorochrome conjugates. Blood samples from 50 RGP-allergic, 41 BV-allergic, and 26 contr..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
The studies were supported financially by a NHMRC Senior Research Fellowship GNT1117687 to MCvZ, a Central Clinical School Early Career Fellowship to CIM, and by NHMRC project grant GNT145303 to PMH, REO'H, BDW.