CytoBas: Precision component-resolved diagnostics for allergy using flow cytometric staining of basophils with recombinant allergen tetramers
Craig I McKenzie, Nirupama Varese, Pei M Aui, Bruce D Wines, Philip Mark Hogarth, Francis Thien, Mark Hew, Jennifer M Rolland, Robyn E O'Hehir, Menno C van Zelm
ALLERGY | WILEY | Published : 2021
BACKGROUND: Diagnostic tests for allergy rely on detecting allergen-specific IgE. Component-resolved diagnostics incorporate multiple defined allergen components to improve the quality of diagnosis and patient care. OBJECTIVE: To develop a new approach for determining sensitization to specific allergen components that utilizes fluorescent protein tetramers for direct staining of IgE on blood basophils by flow cytometry. METHODS: Recombinant forms of Lol p 1 and Lol p 5 proteins from ryegrass pollen (RGP) and Api m 1 from honeybee venom (BV) were produced, biotinylated, and tetramerized with streptavidin-fluorochrome conjugates. Blood samples from 50 RGP-allergic, 41 BV-allergic, and 26 contr..View full abstract
Awarded by NHMRC Senior Research Fellowship
Awarded by NHMRC
The studies were supported financially by a NHMRC Senior Research Fellowship GNT1117687 to MCvZ, a Central Clinical School Early Career Fellowship to CIM, and by NHMRC project grant GNT145303 to PMH, REO'H, BDW.