Journal article

Induction of S-nitrosoglutathione reductase protects root growth from ammonium toxicity by regulating potassium homeostasis in Arabidopsis and rice

Lin Zhang, Haiyan Song, Baohai Li, Meng Wang, Dongwei Di, Xianyong Lin, Herbert J Kronzucker, Weiming Shi, Guangjie Li

JOURNAL OF EXPERIMENTAL BOTANY | OXFORD UNIV PRESS | Published : 2021

Abstract

Ammonium (NH4+) is toxic to root growth in most plants already at moderate levels of supply, but mechanisms of root growth tolerance to NH4+ remain poorly understood. Here, we report that high levels of NH4+ induce nitric oxide (NO) accumulation, while inhibiting potassium (K+) acquisition via SNO1 (sensitive to nitric oxide 1)/SOS4 (salt overly sensitive 4), leading to the arrest of primary root growth. High levels of NH4+ also stimulated the accumulation of GSNOR (S-nitrosoglutathione reductase) in roots. GSNOR overexpression improved root tolerance to NH4+. Loss of GSNOR further induced NO accumulation, increased SNO1/SOS4 activity, and reduced K+ levels in root tissue, enhancing root gro..

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University of Melbourne Researchers

Grants

Awarded by National Key R&D Program of China


Awarded by National Natural Science Foundation of China


Awarded by Natural Science Foundation of Jiangsu Province


Funding Acknowledgements

We thank Dr ElizabethVierling (University of Massachusetts),Dr Xiaofang Zhu (Institute of Soil Science, Chinese Academy of Sciences), and Dr Rongfeng Huang (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) for providing the seeds of proGSNOR:GSNORGFP/hot5-2, hot5-2/gsnor, nia1nia2, and 35S:VTC1-HA/Col-0, and the ABRC of Ohio State University for nox1, noa1, vtc1, and sno1/sos4 mutant seeds. The work was supported by the National Key R&D Program of China (nos 2017YFD0200103 and 2017YFD0200100), the National Natural Science Foundation of China (no. 31972990), the Natural Science Foundation of Jiangsu Province (no. BK20200050), and the University of Melbourne.