Capture of the Circulating Plasmodium falciparum Biomarker HRP2 in a Multiplexed Format, via a Wearable Skin Patch
Khai Tuck Lee, David A Muller, Jacob W Coffey, Kye J Robinson, James S McCarthy, Mark AF Kendall, Simon R Corrie
ANALYTICAL CHEMISTRY | AMER CHEMICAL SOC | Published : 2014
Herein we demonstrate the use of a wearable device that can selectively capture two distinct circulating protein biomarkers (recombinant P. falciparum rPfHRP2 and total IgG) from the intradermal fluid of live mice in situ, for subsequent detection in vitro. The device comprises a microprojection array that, when applied to the skin, penetrates the outer skin layers to interface directly with intradermal fluid. Because of the complexity of the biological fluid being sampled, we investigated the effects of solution conditions on the attachment of capture antibodies, to optimize the assay detection limit both in vitro and on live mice. For detection of the target antigen diluted in 20% serum, i..View full abstract
The authors acknowledge funding from the Australian Research Council (ARC DECRA Fellowship (S.R.C.); Future Fellowship (M.A.F.K)) and the Australian Infectious Diseases Research Centre. We also acknowledge the facilities and the scientific and technical assistance of Prof. Paul Young's research group, the Australian National Fabrication Facility (ANFF Queensland), and the Australian Microscopy & Microanalysis Research Facility (AMMRF) at the Centre for Microscopy and Microanalysis, The University of Queensland.