Clinical signs, pathology and dose-dependent survival of adult wood frogs, Rana sylvatica, inoculated orally with frog virus 3 (Ranavirus sp., Iridoviridae)
Maria J Forzan, Kathleen M Jones, Raphael V Vanderstichel, John Wood, Frederick SB Kibenge, Thijs Kuiken, Wytamma Wirth, Ellen Ariel, Pierre-Yves Daoust
Journal of General Virology | SOC GENERAL MICROBIOLOGY | Published : 2015
Amphibian populations suffer massive mortalities from infection with frog virus 3 FV3, genus Ranavirus, family Iridoviridae, a pathogen also involved in mortalities of fish and reptiles. Experimental oral infection with FV3 in captive-raised adult wood frogs, Rana sylvatica Lithobates sylvaticus, was performed as the first step in establishing a native North American animal model of ranaviral disease to study pathogenesis and host response. Oral dosing was successful LD50 was 10(2.93 2.423.44) p.f.u. for frogs averaging 35mm in length. Onset of clinical signs occurred 614days post-infection p.i. median 11 days p.i. and time to death was 1014 days p.i. median 12 days p.i.. Each tenfold increa..View full abstract
This work was partly funded by the Alexander Graham Bell Graduate Scholarship-Doctoral and the Canadian Cooperative Wildlife Health Centre (now Canadian Wildlife Health Cooperative). The authors thank Dr Alexandra Reid, who kindly provided the isolate of FV3 used in the infection, Drs Marion Desmarchelier, Shannon Martinson and Jonathan Spears, Mr Chris MacQuaid and Maciez Zawadzki, and in particular Dr Jessica Thompson and Ms Sara Vazquez Quiroga, whose efforts were indispensable to the successful rearing of the wood frogs.