Mechanosensing by Peyer's patch stroma regulates lymphocyte migration and mucosal antibody responses
Jonathan E Chang, Matthew B Buechler, Elise Gressier, Shannon J Turley, Michael C Carroll
Nature Immunology | NATURE PUBLISHING GROUP | Published : 2019
Fibroblastic reticular cells (FRCs) and their specialized collagen fibers termed 'conduits' form fundamental structural units supporting lymphoid tissues. In lymph nodes, conduits are known to transport interstitial fluid and small molecules from afferent lymphatics into the nodal parenchyma. However, the immunological contributions of conduit function have remained elusive. Here, we report that intestinal Peyer's patches (PPs) contain a specialized conduit system that directs the flow of water absorbed across the intestinal epithelium. Notably, PP FRCs responded to conduit fluid flow via the mechanosensitive ion channel Piezo1. Disruption of fluid flow or genetic deficiency of Piezo1 on CCL..View full abstract
Awarded by US National Institutes of Health
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
We thank the members of the Carroll laboratory and the Turley laboratory for comments and suggestions on the study. We thank B. Ludewig (Kantonsspital St. Gallen) for providing Ccl19Cre mice. This study was funded in part by Genentech and by the US National Institutes of Health (grant nos. F31 DK1055 to J.E.C.; R33 AI110164 and RO1 AI130307 to M.C.C.; RO1 DK074500 and R21 CA182598 to S.J.T.). We thank S. Jhunjhunwala and N. Lounsbury for management of sequencing files and data processing.