In vitro evaluation of endothelial exosomes as carriers for small interfering ribonucleic acid delivery.
Anna B Banizs, Tao Huang, Kelly Dryden, Stuart S Berr, James R Stone, Robert K Nakamoto, Weibin Shi, Jiang He
International Journal of Nanomedicine | Published : 2014
Exosomes, one subpopulation of nanosize extracellular vesicles derived from multivesicular bodies, ranging from 30 to 150 nm in size, emerged as promising carriers for small interfering ribonucleic acid (siRNA) delivery, as they are capable of transmitting molecular messages between cells through carried small noncoding RNAs, messenger RNAs, deoxyribonucleic acids, and proteins. Endothelial cells are involved in a number of important biological processes, and are a major source of circulating exosomes. In this study, we prepared exosomes from endothelial cells and evaluated their capacity to deliver siRNA into primary endothelial cells. Exosomes were isolated and purified by sequential centr..View full abstract
Awarded by NIDDK NIH HHS
Awarded by NHLBI NIH HHS
Awarded by NCI NIH HHS
Awarded by NIGMS NIH HHS