Journal article

Molecular and functional profiling of apical versus basolateral small extracellular vesicles derived from primary human proximal tubular epithelial cells under inflammatory conditions

Xiangju Wang, Ray Wilkinson, Katrina Kildey, Jacobus PJ Ungerer, Michelle M Hill, Alok K Shah, Ahmed Mohamed, Mriga Dutt, Jeffrey Molendijk, Helen Healy, Andrew J Kassianos

Journal of Extracellular Vesicles | WILEY | Published : 2021

Abstract

Proximal tubular epithelial cells (PTEC) are central players in inflammatory kidney diseases. However, the complex signalling mechanism/s via which polarized PTEC mediate disease progression are poorly understood. Small extracellular vesicles (sEV), including exosomes, are recognized as fundamental components of cellular communication and signalling courtesy of their molecular cargo (lipids, microRNA, proteins). In this study, we examined the molecular content and function of sEV secreted from the apical versus basolateral surfaces of polarized human primary PTEC under inflammatory diseased conditions. PTEC were cultured under normal and inflammatory conditions on Transwell inserts to enable..

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University of Melbourne Researchers

Grants

Awarded by National Health and Medical Research Council


Funding Acknowledgements

Pathology Queensland; Royal Brisbane and Women's Hospital Research Grant; Kidney Research Foundation; National Health and Medical Research Council, Grant/AwardNumber: GNT1099222