Absence of Batf3 reveals a new dimension of cell state heterogeneity within conventional dendritic cells.
Samuel W Lukowski, Inga Rødahl, Samuel Kelly, Meihua Yu, James Gotley, Chenhao Zhou, Susan Millard, Stacey B Andersen, Angelika N Christ, Gabrielle Belz, Ian H Frazer, Janin Chandra
iScience | Elsevier BV | Published : 2021
Conventional dendritic cells (cDCs) are traditionally subdivided into cDC1 and cDC2 lineages. Batf3 is a cDC1-required transcription factor, and we observed that Batf3-/- mice harbor a population of cDC1-like cells co-expressing cDC2-associated surface molecules. Using single-cell RNA sequencing with integrated cell surface protein expression (CITE-seq), we found that Batf3-/- mitotic immature cDC1-like cells showed reduced expression of cDC1 features and increased levels of cDC2 features. In wild type, we also observed a proportion of mature cDC1 cells expressing surface features characteristic to cDC2 and found that overall cDC cell state heterogeneity was mainly driven by developmental st..View full abstract