Mathematical Models for Cell Migration with Real-Time Cell Cycle Dynamics
Sean T Vittadello, Scott W McCue, Gency Gunasingh, Nikolas K Haass, Matthew J Simpson
Biophysical Journal | CELL PRESS | Published : 2018
The fluorescent ubiquitination-based cell cycle indicator, also known as FUCCI, allows the visualization of the G1 and S/G2/M cell cycle phases of individual cells. FUCCI consists of two fluorescent probes, so that cells in the G1 phase fluoresce red and cells in the S/G2/M phase fluoresce green. FUCCI reveals real-time information about cell cycle dynamics of individual cells, and can be used to explore how the cell cycle relates to the location of individual cells, local cell density, and different cellular microenvironments. In particular, FUCCI is used in experimental studies examining cell migration, such as malignant invasion and wound healing. Here we present, to our knowledge, new ma..View full abstract
Awarded by National Health and Medical Research Council
Awarded by Australian Research Council Discovery Program
N.K.H. is a Cameron fellow of the Melanoma and Skin Cancer Research Institute, Australia, and is supported by the National Health and Medical Research Council (AP1084893). M.J.S. is an Australian Research Council Future Fellow, and is supported by the Australian Research Council Discovery Program (DP170100474). S.W.M. is supported by the Australian Research Council Discovery Program (DP140100249).