Journal article

Characterization of the binding site for D-deprenyl in human inflamed synovial membrane

Anna Lesniak, Mikko Aarnio, Shanti Diwakarla, Thomas Norberg, Fred Nyberg, Torsten Gordh

LIFE SCIENCES | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2018

Abstract

AIMS: d-Deprenyl when used as a positron emission tomography tracer visualizes peripheral inflammation. The major aim of the current study was to identify and investigate the properties of the binding target for d-deprenyl in synovial membrane explants from arthritic patients. MAIN METHODS: Thirty patients diagnosed with arthritis or osteoarthritis were enrolled into the study. Homologous and competitive radioligand binding assays utilizing [3H]d-deprenyl were performed to investigate the biochemical characteristics of the binding site and assess differences in the binding profile in synovial membranes exhibiting varying levels of inflammation. KEY FINDINGS: The [3H]d-deprenyl binding assay ..

View full abstract

University of Melbourne Researchers