Characterization of the binding site for D-deprenyl in human inflamed synovial membrane
Anna Lesniak, Mikko Aarnio, Shanti Diwakarla, Thomas Norberg, Fred Nyberg, Torsten Gordh
LIFE SCIENCES | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2018
AIMS: d-Deprenyl when used as a positron emission tomography tracer visualizes peripheral inflammation. The major aim of the current study was to identify and investigate the properties of the binding target for d-deprenyl in synovial membrane explants from arthritic patients. MAIN METHODS: Thirty patients diagnosed with arthritis or osteoarthritis were enrolled into the study. Homologous and competitive radioligand binding assays utilizing [3H]d-deprenyl were performed to investigate the biochemical characteristics of the binding site and assess differences in the binding profile in synovial membranes exhibiting varying levels of inflammation. KEY FINDINGS: The [3H]d-deprenyl binding assay ..View full abstract
Awarded by Swedish Medical Research Council
This work was supported by the Berzelii Technology Centre for Neurodiagnostics and the Swedish Medical Research Council [Grant 9459]. We thank Dr. Jan Millbrink and Ms. Maria Essermark for providing synovial membrane samples.